Major Glenn Talbot was brought to Desert Base, New Mexico by its commanding officer, General Thaddeus E. "Thunderbolt" Ross, for two reasons. First, Ross wanted Talbot, who was the base's new security chief, to investigate nuclear physicist Dr. Robert Bruce Banner, who had been working on various government projects at the base. Due to Banner's continuous and mysterious disappearances, Ross suspected not only that Banner was a traitor but also that he had some connection with the monstrous Hulk, who prowled the vicinity. Secondly, Ross objected to his daughter love for Banner, whom Ross believed to be unmanly even if he was not a traitor. Ross hoped that Betty would instead fall in love with Talbot, whom the general regarded as a fine example of a military man.
Talbot, now General Ross's adjutant, continued to help Ross attempt to capture or destroy the Hulk, whom they regarded as a menace. At the time, Banner was unable to maintain his own intelligence and personality when he transformed into the Hulk. Eventually, though, he found a means by which his own persona could maintain control of the Hulk when he transformed into that monstrous being. It appeared that the menace of the Hulk was over, and General Ross reluctantly consented to the wedding of Bruce Banner and his daughter. But the wedding ceremony was ruined when, before Banner and Betty Ross were officially joined in matrimony, the Hulk's arch enemy, the Leader, transformed Banner back into the savage Hulk of old.
Thinking that Banner was forever lost to her as a potential husband, Betty Ross eventually married Glenn Talbot. Both Talbot and General Ross believed that Betty actually still loved Banner, although Betty herself believed that she truly loved Talbot. Nevertheless, Talbot and General Ross were correct.
With the permission of the Canadian government, General Ross and his Hulkbusters pursued the Hulk through Canada into the Arctic. Ross was taken prisoner by the Gremlin, who then headed a secret Soviet base that had been established there. The general was sent to a prison in the Soviet Union and Talbot volunteered to help rescue him. Although Ross was freed, Talbot himself was shot during the rescue mission and believed to be dead. Betty blamed her father for Talbot's reported demise.
In actuality, however, Talbot was still alive and being held prisoner by the Soviets at Bitterfrost, a top secret Soviet installation in Siberia. A man named Gregori Kronski was subjected to surgery that made him a double of Talbot. The Gremlin used his Psiclone device to imprint the memories and personality of Talbot upon Kronski's mind, and those of Kronski upon Talbot's. Kronski was then sent to the United States, claiming to be an escaped Talbot. However, a bomb was concealed within Kronski's body. The Gremlin's plan was to use the bomb to assassinate the President of the United States when the latter came to visit Gamma Base, where Talbot was stationed. Colonel Jack D. Armbruster saved the President's life, but both Armbruster and Kronski were killed when the bomb exploded.
General Ross then accompanied a team of SHIELD agents led by Clay Quartermain into the Soviet Union to rescue the real Talbot. With assistance from the Hulk, Ross and Quartermain succeeded in freeing Talbot, who believed himself to be Kronski, and returned with him to the United States. There, Talbot's true personality and memories were restored.
Talbot learned to his dismay that Betty no longer believed herself to be in love with him, and that she realized she had never stopped loving Banner. Glenn and Betty Talbot were therefore soon received a divorce.
The strain brought on by his many years of failures at ending the menace of the Hulk caused General Ross to suffer a nervous breakdown. He was relieved of command of Gamma Base, and Talbot, as the man with the next most experienced battling the Hulk, was promoted to colonel and given command of Gamma Base by the Pentagon. Determined to gain vengeance for the loss of his wife's love and for Ross's nervous collapse, Talbot was intent on destroying the Hulk.
Talbot though, proved no more successful at destroying the Hulk than Ross had been. Dissatisfied with Gamma Base's repeated failures to end the menace of the Hulk, Congress finally shut the base down. Now obsessed with killing the Hulk, Talbot committed treason by stealing the War Wagon, a highly sophisticated flying battle cruiser, and went off on his own to locate and kill the Hulk.
Talbot finally caught up with the Hulk at Mount Kuroishi, an inactive volcano, in Japan. Talbot launched a massive onslaught against the Hulk using the War Wagon's immensely powerful arsenal of weaponry. The battle triggered an earthquake that reactivated the volcano. During the fight Talbot wore an encephalo-helmet that enabled him to take direct mental command of the War Wagon's controls (which ordinarily were run by on-board computers). Talbot bombarded the Hulk with intense sonic beams, causing the monster tremendous pain. The Hulk then struck the mountain so hard that its cone was shattered and a torrent of lava, ash, and super-heated air struck and covered the hovering War Wagon. The heat and force that thus struck the War Wagon caused an electrical overload in its systems that traveled through the encephalo-helmet and incinerated Talbot's brain. Talbot died instantly, and with no one to control the War Wagon, the flying vehicle crashed to Earth. The Hulk escaped the scene, having suffered no permanent injury; Talbot had failed to stop the Hulk for the final time, this time at the price of his own life.